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Issue Info: 
  • Year: 

    2024
  • Volume: 

    21
  • Issue: 

    150
  • Pages: 

    192-205
Measures: 
  • Citations: 

    0
  • Views: 

    22
  • Downloads: 

    0
Abstract: 

Escherichia coli and Staphylococcus aureus are pathogens that have the ability to form biofilms and cause disease in food products. Due to the fact that the enterotoxins produced by these two pathogens remain in a wide range of temperature, pH and saline conditions, they cause severe infections in humans. Melittin is a natural peptide derived from bee venom that can show its antimicrobial and anti-biofilm potential through disrupting the membrane of bacterial cells. For this purpose, in this study, the antimicrobial effect of this peptide on Gram positive and negative bacteria was investigated and its minimum inhibitory concentration (MIC) was determined as 100 µg/mL and 300 µg/mL, respectively. Also, the scanning Electron Microscope images confirmed the antimicrobial effect of the peptide on these two bacteria. Peptide melittin caused wrinkling, deformation and creation of holes in the cell membrane of treated bacteria, compared to the control sample. On the other hand, the results of the biofilm inhibition test showed that the addition of the peptide at a concentration of 2MIC completely prevented the biofilm formation of S. aureus prevented, while this value was equal to 91.00 ± 2.82 in E. coli bacteria. Also, the increase in peptide concentration caused an increase in the destruction of adult biofilms of both bacteria. On the other hand, this peptide decreased the invasion and adhesion of these two bacteria to HT-29 and Caco-2 cells by reducing the mobility of pathogens. Therefore, according to the obtained results, melittin peptide can be a suitable alternative to chemical disinfectants that are harmful to the environment.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2006
  • Volume: 

    1
  • Issue: 

    1
  • Pages: 

    15-17
Measures: 
  • Citations: 

    0
  • Views: 

    298
  • Downloads: 

    134
Abstract: 

Background: Due to repeated cholera outbreaks in Iran and neighboring countries, the present study was performed to determine the prevalence of phenotypes of Ogawa and Inaba serotypes of Vibrio cholera 01 and classic Vibrio cholera 01 biotypes.Materials and methods: Scanning and transmission Electron microscopy (SEM and TEM) were applied on 4 species, of Ogawa and Inaba serotypes and two classic serotypes of Vibrio cholera 01. Results: Membrane diameter of Eltor was wider as compared to classic biotype. Number of ribosomes, protein synthesis, length and number of flagella were quite more in comparison with classic biotype. Conclusion: According to our findings, genome of classic biotype is more compact.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Author(s): 

RIS H.A.

Issue Info: 
  • Year: 

    1956
  • Volume: 

    2
  • Issue: 

    4
  • Pages: 

    385-391
Measures: 
  • Citations: 

    1
  • Views: 

    95
  • Downloads: 

    0
Keywords: 
Abstract: 

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Author(s): 

Shobeikeh Sanaz

Issue Info: 
  • Year: 

    2019
  • Volume: 

    6
  • Issue: 

    4 (26)
  • Pages: 

    32-42
Measures: 
  • Citations: 

    0
  • Views: 

    302
  • Downloads: 

    0
Abstract: 

For many years now, Conventional Transmission Electron Microscope (CTEM) has been used as an efficient tool to fulfill Nano scale analysis and imaging requirements of scientists and researchers of different areas. Despite the fact that CTEM is a very efficient tool, in some areas researchers must add specially designed equipment to this Microscope in order to create desired conditions for observing and imaging their specific specimens. In this article, we introduce one of the most practical kind of CTEM that has been invented for over a decade namely, Energy-Filtering Transmission Electron Microscope (EFTEM). EFTEM has been designed by using physical principles of Electron energy-loss spectrometry (EELS) and commercial in-column or post-column energy-filters introduced by companies like: ZEISS and GATAN. It can generate precise images from ultrastructure of different specimens belonging to different areas of research by using inelastically scattered Electrons. The operation of these kind of energy-filters is straightforward and because of that acquiring element specific images within only a few minutes has become possible. For obtaining best results, a fundamental knowledge of the underlying physics of EELS and a systematic development of the technical details is necessary. Also it is important that users be able to know with certainty that EFTEM is the optimal equipment for their experiments. For aforementioned reason, detailed structures of available energy filters and applications of EFTEM in various fields are reviewed in the next article titled “ Introduction to Energy-Filtering Transmission Electron Microscope structure and applications” that will be publish in the next issue of this journal.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2025
  • Volume: 

    22
  • Issue: 

    1
  • Pages: 

    71-82
Measures: 
  • Citations: 

    0
  • Views: 

    9
  • Downloads: 

    0
Abstract: 

The properties of steels are intrinsically dependent on their microstructural components, known as phases, which form during the manufacturing process. Different steel phases can be observed in microscopic images of steel surfaces. Automatic detection and classification of these phases from images can significantly enhance the understanding of steel properties with improved speed and accuracy. This paper introduces, for the first time, an intelligent and automated method for classifying steel phases from microscopic images. This process requires defining and extracting suitable texture features unique to these images and segmenting the images into highly irregular regions based on the extracted features. To achieve this, the input image is initially divided into blocks, and texture features are extracted independently for each block. The dimensionality of these features is then reduced using Principal Component Analysis, and the refined features are subsequently fed into a Softmax neural network for classification. The implementation results indicate that the proposed method achieves an accuracy of over 99% in distinguishing between two phases: acicular ferrite and granular ferrite. Furthermore, it attains an accuracy exceeding 86% when classifying three phases: granular ferrite, acicular ferrite, and Widmanstätten ferrite. This suggests that the widely used and conventional k-means clustering method, as a traditional machine learning approach, is incapable of effectively distinguishing microscopic steel phase blocks using extracted texture features. Notably, as of the writing of this paper, no prior research has been conducted on the automatic classification of different ferrite phases, making this study a novel contribution to the field. In this research, an automated classification algorithm for ferrite phase structures in SEM images of steel is proposed using texture feature extraction methods and machine learning models. The dataset comprises images of 1024×768 resolution, which were divided into 128×128 blocks, with classification performed independently for each block. Due to the limited number of blocks available for training machine learning models, data augmentation techniques such as rotation and scaling were applied to increase the dataset size. Various image processing methods were used to extract 128 texture features. These extracted features were then used to classify different ferrite phases using two machine learning models: k-means clustering and the Softmax neural network. Additionally, PCA was employed to reduce feature dimensionality, which positively impacted the classification of granular and acicular ferrite. While k-means clustering, as a conventional and widely used machine learning method, failed to achieve satisfactory classification accuracy, the proposed approach using a smooth maximum neural network demonstrated exceptional performance. Despite the complex and irregular nature of ferrite shapes, the selected features and the proposed algorithm successfully achieved over 99% accuracy for two-phase classification and over 86% accuracy for three-phase classification.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Author(s): 

BANCROFT M. | BELLAIRS R.

Issue Info: 
  • Year: 

    1997
  • Volume: 

    151
  • Issue: 

    -
  • Pages: 

    97-108
Measures: 
  • Citations: 

    1
  • Views: 

    78
  • Downloads: 

    0
Keywords: 
Abstract: 

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 78

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Author(s): 

Shobeikeh Sanaz

Issue Info: 
  • Year: 

    2019
  • Volume: 

    7
  • Issue: 

    1 (25)
  • Pages: 

    27-37
Measures: 
  • Citations: 

    0
  • Views: 

    1828
  • Downloads: 

    0
Abstract: 

A comprehensive background information concerning underlying basis of final image formation procedure inside an Energy-Filtering Transmission Electron Microscope (EFTEM) has been provided in our previous article titled “ Introduction to Energy-Filtering Transmission Electron Microscope fundamental principles” (published in Iranian Journal of Laboratory knowledge; Volume 6; Issue 4; Winter 2019; No. 24). In this article, structures of two widely used commercial energy filters have been reviewed in detail, namely, In-Column Filters and Post-Column Filters. . In-Column Filters and Post-Column Filters are located inside and beneath a Conventional Transmission Electron Microscope (CTEM), respectively. It is very important that users in various fields know when to use this piece of valuable equipment to get the desired results. For this purpose, applications of Energy-Filtering Transmission Electron Microscope in various fields like: Biology, Polymer science, Semiconductors, and Carbon nanotubes has been explained with simple examples in order to obtain a better comprehension of the unique operations and advantages of this equipment.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2004
  • Volume: 

    22
  • Issue: 

    73
  • Pages: 

    27-30
Measures: 
  • Citations: 

    0
  • Views: 

    824
  • Downloads: 

    0
Keywords: 
Abstract: 

Introduction. The lumen of the vascular system is covered by a continuous layer of endothelial cells, and any changes or damages to them is due to cardiovascular diseases especially atherosclerosis. Lead is one of the elements which may cause endothelial dysfunction. The purpose of this study was to determine the effect of lead on morphologic features of endothelium which may induce the endothelial dysfunction.Methods. Twenty white male rabbits divided randomly into two groups. Group A (n=11) were given regular diet with leaded water (547 ppm) and group B (n=9) were fed with regular diet and unlead water for 40 days. The animals were sacrificed and their aorta, left cornorary and middle cerebral arteries were dissected and morphological changes of endothelial cells studied by SEM.Results. Scanning Electron microscopic study of endothelial cells of aorta, left coronary and middle cerebral arteries that received lead showed morphological changes. In aorta disruption and denuation of endothelial cells, exposure of the subendothelial layer and adhesion of red blood cells, leukocytes and platelets on surface of endothelium, and presence of craters showing disrupted surface was observed. Similary endothelial cells of left coronary and middle cerebral arteries were found to show irregular orientation.Discussion. Morphologic changes of endothelial cells of aorta demonstrated that lead can cause dysfunction. In coronary and middle cerebral arteries very limited morphological changes was observed.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Issue Info: 
  • Year: 

    2014
  • Volume: 

    5
  • Issue: 

    -
  • Pages: 

    133-140
Measures: 
  • Citations: 

    1
  • Views: 

    101
  • Downloads: 

    0
Keywords: 
Abstract: 

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View 101

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Issue Info: 
  • Year: 

    2019
  • Volume: 

    7
  • Issue: 

    1 (25)
  • Pages: 

    13-26
Measures: 
  • Citations: 

    0
  • Views: 

    2894
  • Downloads: 

    0
Abstract: 

Electron Microscope is one of the best methods of analysis that is widely used in various fields. The Microscope provides the possibility of examining the surface and microstructure in micron and nano dimensions. Today, one of the most specialized areas of interest is biological and living tissue. The observation of these samples and the investigation of their microstructures by scanning Electron Microscope require special preparation. Due to the high sensitivity of the samples, if any of the steps in the preparation are not carefully performed, it will cause shrinkage, collapse, or damage to the sample structure and will affect the quality of the final images, so it is very important that the conditions and procedures for tissue preparation carefully done with the standard method. There are many different techniques for preparing and analyzing various types of tissue that vary according to the type of tissue and structure. This paper study the general methods for the preparation of biological samples and living tissues including: a) various fixations (chemical fixtaion and physical fixation), (b) dehydration with different percentages of alcohol, and (c) different drying methods In air, critical point dryers, freeze dryers and chemical dryers), in order to view the biological structures in the closest to their living structure in a high resolution SEM is discussed.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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